CATH 3.40.50.620 / FunFam 145 · diphthine—ammonia ligase
How the pipeline reads a protein family
Every step: what goes in → which tool → what comes out → what it tells us. Follow the rail top to bottom.
conserved (high scorecons)
predicted pocket (P2Rank)
functional core (both)
variable / gap
What the family turns into
47→41
members after cleanup
11
catalytic-core residues
1
archaea-specific site (Q50H)
From 47 database IDs with mostly-missing labels, the pipeline ends at a specific, testable claim:
11 residues that are both evolutionarily conserved and sit in the predicted ATP pocket — the enzyme's likely active site — plus one residue (50) that flips from Gln to His only in archaea.