CATH 3.40.50.620 / FunFam 145 · diphthine—ammonia ligase

How the pipeline reads a protein family Every step: what goes in → which tool → what comes out → what it tells us. Follow the rail top to bottom.

conserved (high scorecons) predicted pocket (P2Rank) functional core (both) variable / gap

What the family turns into

47→41
members after cleanup
11
catalytic-core residues
26
un-annotated, rescued
1
archaea-specific site (Q50H)

From 47 database IDs with mostly-missing labels, the pipeline ends at a specific, testable claim: 11 residues that are both evolutionarily conserved and sit in the predicted ATP pocket — the enzyme's likely active site — plus one residue (50) that flips from Gln to His only in archaea.